Induced Pluripotent Stem Cells Essays - 1185 Words

Describe:- (i) how induced pluripotent stem cells (iPSCs) are isolated (ii) how iPSCs can be used to study human development (iii) how iPSCs can be used to model human disease and to develop therapies for degenerative disease i) iPSCs are adult stem cells that have been genetically reprogrammed to behave like the pluripotent stem cells found in embryos, i.e. can differentiate into any cell type in the human body. This was first completed successfully in mice in 2006 by Shinya Yamanaka and his team (Takahashi et al., 2006), then in humans in 2007 both by Yamanaka (Takahashi et al., 2007), and by James Thomson and his team in America independently (Yu, et al., 2007). Yamanaka and Thomson’s methods were similar. In the report by Yu et†¦show more content†¦964-965). ii) Human embryonic stem cells (ESCs) are pluripotent cells isolated from blastocysts, and are highly useful in studying human development (Itzkovitz-Eldor et al., 2000 p. 88). Although the National Institute of Health states that â€Å"it is not known if iPSCs and embryonic stem cells differ in clinically significant ways†, iPSCs are already being used to achieve the same results as ESCs in some applications without the use of embryos, removing the ethical concern associated with ESCs (National Institutes of Health, 2009). ESCs are capable of differentiating into all cell types, and can be used as a source of differentiated cells. In the report by Itskovitz-Eldor et al., they discuss the induced differentiation of ESCs in suspension into embryoid bodies, including the three embryonic germ layers. The authors state that â€Å"the ability to induce formation of human embryoid bodies that contain cells of neuronal, hematopoietic and cardiac origins will be useful in studying ea rly human embryonic development† (Itzkovitz-Eldor et al., 2000 p. 88). This is invaluable in terms of studying the early stages of human foetal development. As iPSCs are better understood, and the methods of generating them are refined, they have the potential to play an increasingly integral role in our study of human development, in place of ESCs. In the report by Itzkovitz-Eldor et al. it is noted that the abilityShow MoreRelatedProgress in the Reasearch on Induced Pluripotent Stem Cells 528 Words   |  3 Pagesstudy in stem cell research. They reprogrammed mouse skin fibroblasts by introduction of four transcription factors, Oct3/4, Sox2, Klf4 and c-Myc and generated cells almost indistinguishable from ES cells. They named these cells induced pluripotent stem cells (iPSCs) (Takahashi K., et al., 2006). An year later, James A. Thomson et al. replaced Oct4 and oncogenic c-Myc with Lin28 and Nanog decreasing the risk of cancer formation (Yu J., et al., 2007). We need to take into consideration that cell typesRead MoreAnalysis Of Adipose Derived Induced Pluripotent Stem Cells4532 Words   |  19 PagesAnalysis of adipose derived induced pluripotent stem cells (iPSC’s) microRNA (miRNA) expression profile over multiple passages and pancreatic ÃŽ ²-cell miRNA expression profile post differentiation from iPSC’s. Simplified title Improvement of stem cell differentiation to pancreatic ÃŽ ²-cell for transplantation into diabetics. 2) Project summary Ethical concerns regarding the use of embryo-derived embryonic stem cells (ES), and the immune response elicited by differentiated ES cells, are being circumventedRead MoreCan Skin Dermal Papilla (DP) Cells Differentiate into Induced Pluripotent Stem Cells?578 Words   |  3 Pages(DP) cells can differentiate into induced pluripotent stem cells (iPSCs) by using the transcription factor, OCT4, instead of using all four transcription factor, OCT4, Sox2, Klf4, and c-Myc, which are usually used to differentiate somatic cells into stem cells. The scientists came up with the question because two out of the four transcription factors, Klf4 and c-Myc, are oncogenic gene, thereby it is best to replace these genes with other safer alternatives. Reprogramming cells into induced pluripotentRead MoreInduced Pluripotent Stem Cells Essay1759 Words   |  8 Pages Induced Pluripotent Stem Cells: Methods of Production, Characterization and Applications to Medicine Student’s Name University Induced Pluripotent Stem Cells: Methods of Production, Characterization and Applications to Medicine Introduction By definition, induced pluripotent stem cells (iPSCs) are somatic cells with preprogrammed features (Kamath et al. 2017). In essence, they are often modified to mimic embryonic stem cells through the expression of ectopic factors linked to geneRead MoreInduced Pluripotent Stem Cells And The Maintenance Of Neural Crest Stem Cell1331 Words   |  6 Pages Induced Pluripotent Stem Cells—Derived Neural Crest Stem Cells and the maintenance of Neural crest stem cell multipotency Stem cells are unspecialized cells, which have the potential to develop into several different cell types—muscle cells, brain cells, skin cells, or red blood cells—during early development. Stem cells continuously divide in order to replace other cells. As stem cells divide, they have the potential to develop into a specialized cell or remain a stem cell. Induced pluripotentRead MoreDescribe How Mature Somatic Cells Can Be Reprogrammed to Become Pluripotent991 Words   |  4 Pagestechnology, it is possible to reprogram matured somatic cells in pluripotent cells by introducing factors that can induce cell in situ reprogramming. (1) This technique is highly useful in medicine, including acting as a model for drug and clinical testing, development of drugs. Pluripotent stem cells are the stem cells that can only differentiate into a limited range of differentiated cells. (2) They have the ability to give rise to all somatic cells from ectoderm, mesoderm and endoderm, as well as gametesRead MoreA Research Study On Stem Cell Research1324 Words   |  6 Pageslike this. He knows that stem cells could help cure him from his misery, so he has been fighting and fighting for stem cell research to be fully funded in America. Stem cells are cells that have the skill to change into any type of cell in the human body (Lewis). These cells could be put anywhere ill in the body to make it healthy again. Imagine all of the people with chronicle diseases and cancer being able to have hope for being happy and healthy again. Also, these stem cells can fix damaged organsRead MoreBiotechnology: A Tool of Body Manipulation Essay896 Words   |  4 PagesSum, 2013. In the public performance the artist donated her skin cells for transformation through iPSC technology into induced pluripotent stem cells (also known as iPS cells or iPSCs). Challenging the existing ethical dilemma around embryonic cell research, she allowed her adult cells to be then transformed into blood, brain and heart cells. As the creation of her second self outside once body, the artist exhibited the living cells then in the gallery space exercising the non-fixed and non-givenRead MoreStem Cell Research : Ethics, Cloning And Curing The Disease1220 Words   |  5 PagesOutline Stem Cell Research - Ethics, Cloning and Curing the Disease Introduction According to former Speaker of the House, John Boehner, †Stem cell research must be carried out in an ethical manner in a way that respects the sanctity of human life.† In recent events, stem cell research has caught the attention of the nation and stirred up controversy about the research and ethics along with it. Ethically, stem cell research has caught more attention than the research behind it. Stem cell researchRead MoreThe Debate Over Embryonic Stem Cell Research1710 Words   |  7 PagesEmbryonic Stem Cell Research Embryonic stem cells, according to the National Institute of Health, are cells obtained from embryos that are prepared from fertilized eggs which have been collected through the in vitro technique; in specialized clinics. Scientists use the stem cell research to have a better idea about the evolution, aging, and disease. Thus, they keep track of the stem cells progress across the lifetime; this tracking process can help scientists to figure out the genetic mutations